Glycoengineered extracellular vesicles released from antibacterial hydrogel facilitate diabetic wound healing by promoting angiogenesis

• DOI: 10.1002/jev2.70013
• Publication Date: 2024-11-27T08:38:57Z
• Authors: Kewei Wang; Qin He; Mengmeng Yang; Qincheng Qiao; Jun Chen; Jia Song; Nan Zang; Huiqing Hu; Longqing Xia; Yingyue Xiang; Fei Yan; Xinguo Hou; Li Chen
• Keywords: Granulation tissue; Biocompatibility; Lipofectamine

Abstract Diabetic wounds have become a global healthcare burden owing to impaired angiogenesis and persistent infections. Extracellular vesicles (EVs) can improve diabetic wounds, though their targeting ability is limited. In this study, we investigated the performance of novel hydrogel dressing comprised gelatin methacryloyl, glycoengineered EVs, polylysine in treating infected wounds. High‐throughput single‐cell RNA sequencing (scRNA‐seq) immunofluorescence staining revealed that E‐selectin (SELE) levels were higher than non‐diabetic Mesenchymal stromal cells (MSCs) transfected with lentivirus containing fucosyltransferase VII (FUT7) CD63‐P19‐Nluc vector enhance expression sialyl Lewis X (sLeX), ligand E‐selectin, on surface EVs (s‐EVs) derived from MSCs (s‐MSCs). s‐EVs target human umbilical vein endothelial (HUVECs) under lipopolysaccharide stimulation promote function stimulated HUVECs vitro. To sustain release s‐EVs, fabricated methacryloyl (Gel)/poly‐L‐lysine (PL)‐5 good antibacterial ability, biocompatibility mechanical properties. mouse experiment, s‐EV@Gel/PL‐5 exhibited excellent anti‐inflammatory abilities further promoted healing Our findings demonstrated potential clinical treatment infectious