Leukotriene B4-activated human endothelial cells promote transendothelial neutrophil migration

• DOI: 10.1002/jlb.62.2.203
• Publication Date: 2018-01-03T17:58:23Z
• Authors: Masaharu Nohgawa; Masataka Sasada; Akinori Maeda; Kohsuke Asagoe; Nari Harakawa; Kuniko Takano; Kokichi Yamamoto; Minoru Okuma
• Keywords: Leukotriene B4; Extravasation; N-Formylmethionine leucyl-phenylalanine

We explored the effect of leukotriene B4 (LTB4) on endothelial cells in LTB4-induced transendothelial migration (TEM) neutrophils as an vitro model neutrophil extravasation. Chemotactic response human to LTB4 was significantly lower than that N-formyl-methionyl-leucyl-phenylalanine (fMLP), whereas extent TEM higher fMLP. The study random induced by and fMLP also showed similar results, which indicated might affect umbilical cord vein cell (HUVEC) barrier. Neutrophil pretreatment HUVEC monolayer with but not Treatment ONO-4057, a receptor antagonist, abolished almost completely treated ONO-4057 could transmigrate through LTB4. These findings induce acting HUVEC.