Tuning compounds for electrospray ionization/in‐source collision‐induced dissociation and mass spectra library searching

• DOI: 10.1002/jms.201
• Publication Date: 2002-08-25T20:44:49Z
• Authors: Wolfgang Weinmann; Mylène Stoertzel; Susanne Vogt; Michal Svoboda; André Schreiber
• Keywords: Fragmentation

Tuning compounds for positive and negative electrospray ionization (ESI) were tested the tuning of in-source collision-induced dissociation (ESI/CID) with three types SCIEX API instruments (API 365, 2000 3000) in single-quadrupole mode. The vacuum interfaces these differ slightly geometry, but principles solvent evaporation by nebulizer curtain gases, orifice skimmer are identical. For comparison CID, breakdown curves haloperidol, paracetamol, metronidazole metamizole acquired increasing voltages. 3000 required higher voltages than did 365 to induce a similar degree fragmentation protonated or deprotonated molecules characteristic fragment ions. This increase voltage could be demonstrated each four shift maxima A procedure collision energy (CE) levels drug identification mass spectra library set up an therefore adjustment values when being transferred 3000. corresponding 20/50/80 V 365), 30/90/130 2000) 40/80/120 3000). However, change +/-10 (with hardly influenced fit search single CE level. adjusting different instruments, haloperidol paracetamol is presented. With this ESI/CID 150 also used good results.