The autotomy relief effect of a silicone tube covering the proximal nerve stump

• DOI: 10.1002/jor.20196
• Publication Date: 2006-05-26T22:28:06Z
• Authors: Teruaki Okuda; Osamu Ishida; Yoshinori Fujimoto; Nobuhiro Tanaka; Atsuko Inoue; Yoshihiro Nakata; Mitsuo Ochi
• Keywords: Autotomy; Neuroma

A technique of covering the proximal nerve stump (PNS) has been reported as a preventative method or treatment for neuroma. However, its detailed pain relief mechanism remains unknown. We created silicone tube model in which PNS rat sciatic was introduced into tube, whereas controls had no tube. The score autotomy observed group lower than that control at 3 days to 2 weeks after surgery, suggested pain-like behavior inhibitory action. To elucidate inhibition, immunohistochemistry and Toluidine blue staining were performed PNS. increase S-100-immunoreactivity (IR) including Schwann cells inhibited 1 week surgery group, number macrophages shown by ED-1-IR 1, 2, 4 similarly inhibited. showed mast lymphocytes group. Therefore, blocking infiltration inflammatory thought be inhibition. further explore details this mechanism, expression growth factor (NGF), production is induced NGF receptor TrkA examined dorsal root ganglion using ribonuclease protection assay. In PNS, NGF-IR suggesting could one effects. © 2006 Orthopaedic Research Society. Published Wiley Periodicals, Inc. J Orthop Res 24:1427–1437,