High fat diet accelerates cartilage repair in DBA/1 mice

• DOI: 10.1002/jor.23280
• Publication Date: 2016-05-05T01:34:32Z
• Authors: Wu Wei; Yvonne M. Bastiaa...; Mathijs Suijkerbuijk; Nicole Kops; Pieter K. Bos; Jan A.N. Verhaar; Anne-Marie Zuurmond; Francesco Dell'Accio; Gerjo J.V.M. van ...
• Keywords: Histology

Obesity is a well-known risk factor for osteoarthritis, but it unknown what does on cartilage repair. Here we investigated whether high fat diet (HFD) influences repair in mouse model of We fed DBA/1 mice control or HFD (60% energy from fat). After 2 weeks, full thickness defect was made the trochlear groove. Mice were sacrificed, 1, 8, and 24 weeks after operation. Cartilage evaluated histology. Serum glucose, insulin amyloid A measured h before operation at endpoints. Immunohistochemical staining performed synovium adipose tissue to evaluate macrophage infiltration phenotype. One week operation, had filling with fibroblast-like cells more as indicated by lower Pineda score. 8 still no complete did not detect significant difference between diets. Bodyweight increased HFD, whereas serum influenced. Macrophage phenotype influenced HFD. In contrast common wisdom, accelerated intrinsic short term. Resistance induced inflammatory metabolic changes could be associated © 2017 Orthopaedic Research Society. Published Wiley Periodicals, Inc. J Orthop Res 35:1258-1264, 2017.