Biologic response of human anterior cruciate ligamentocytes on collagen‐patches to platelet‐rich plasma formulations with and without leucocytes

• DOI: 10.1002/jor.23599
• Publication Date: 2017-05-09T12:10:48Z
• Authors: Anna M. Krismer; Romina S. Cabra; Rahel D. May; Daniela A. Frauch...; Sandro Kohl; Sufian S. Ahmad; Benjamin Gantenbein
• Keywords: Catabolism; Matrix (chemical analysis)

Due to the poor self-healing capacities of anterior cruciate ligament, previous primary repair attempts have failed. To enhance biologic healing, platelet rich plasma and collagen scaffold shown promise in animal models. Platelet (PRP) is already used several clinical applications although outcomes are quite debated. The purpose this study was examine effects different PRP formulations during 21 days: With leucocytes pure on human ligament-derived ligamentocytes grown patches 3D cell cultures vitro. Three experimental groups were formed: 2.5% leucocyte PRP, 20% a negative control, positive control. Cell proliferation, phenotype mRNA transcript level, extracellular matrix production (total glycosaminoglycan content) evaluated. DNA content metabolic activity increased significantly all day compared 7, except No changes detected. Different catabolic genes induced depending concentration PRP. with without treated proliferation but not production. However, specific activation dependent relative leucocytes. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:2733-2739, 2017.