Angiopoietin‐like protein 2 deficiency promotes periodontal inflammation and alveolar bone loss

• DOI: 10.1002/jper.21-0290
• Publication Date: 2021-10-28T12:40:55Z
• Authors: Chenxi Jiang; Siqi Yao; Yi Guo; Li Ma; Xiaoxuan Wang; Yuan Chen; Huihui Zhang; Zhengguo Cao
• Keywords: Periodontal fiber

Abstract Background Human periodontitis is a highly prevalent inflammatory disease that leads to connective tissue degradation, alveolar bone resorption, and tooth loss. Angiopoietin‐like 2 (ANGPTL2) regulates chronic inflammation in various diseases functionally involved maintaining homeostasis promoting regeneration, but there limited information about its function periodontitis. Here we investigated the expression explicit role of ANGPTL2 Methods Immunohistochemistry quantitative real‐time PCR (qRT‐PCR) were used detect periodontal tissues ligament cells (PDLCs). A ligature‐induced model was generated wild‐type knockout mice. qRT‐PCR enzyme‐linked immunosorbent assay assess production cytokines matrix metalloproteinases (MMPs) cultured PDLCs. Western blot performed proteins relevant signaling pathways. Results Increased observed inflamed deficiency promoted loss with enhanced osteoclastogenesis reactions Downregulation remarkably levels interleukin (IL)‐6, IL‐8, MMP1, MMP13 Porphyromonas gingivalis lipopolysaccharide‐induced PDLCs, whereas ANGPTL2‐overexpressing PDLCs showed opposite trends. downregulation activated STAT3 nuclear factor‐κB pathways blocked Akt under environment. Treatment inhibitor partially suppressed reaction mediated by knockdown. Conclusions Our study provides first evidence an anti‐inflammatory effect murine The findings demonstrate critical protective inflammation.