A reporter assay for the next generation of biomaterials: Porous‐wall hollow glass microspheres

• DOI: 10.1002/lary.24414
• Publication Date: 2013-10-12T01:40:11Z
• Authors: Aaron Cunningham; Hunter Faircloth; Matthew Jones; Christopher Johnson; Tiffany Coleman; George Wicks; Gregory Postma; Paul Weinberger
• Keywords: Biomaterial

Objectives/Hypothesis The primary objective was to design a reporter assay measure molecular release kinetics from new porous‐wall hollow glass microsphere biomaterial with great potential in regenerative medicine and drug delivery. Second, future avenues for research will be discussed specifically regard clinical uses laryngology. Study Design Basic science data report. Methods We developed an using fluorescent nanocrystals or quantum dots (Qdot 605) as reporter. A Nuance FX multispectral imaging system used detect fluorescence aqueous phase. Spectral output of known concentrations Qdot 605 measured by the create standard curve. Results These were plotted fit emission demonstrates excellent correlation concentration log‐log relationship [ R 2 = 0.99649, median error 9.9%], indicating that is reliable should explored regarding its ability evaluate drug‐eluting properties this material. Conclusions have derived method microscopy, which facilitate on exciting biomaterial. This material has use head neck surgery. Specific within laryngology investigated include laryngeal tracheal reconstruction, vocal fold healing, nerve regeneration. Furthermore, we believe first documented determine concentrations. Level Evidence NA Laryngoscope , 124:1392–1397, 2014