Chondroitin and Dermatan Sulfate Bioinks for 3D Bioprinting and Cartilage Regeneration

• DOI: 10.1002/mabi.202100435
• Publication Date: 2022-01-14T05:59:33Z
• Authors: Markel Lafuente‐M...; Sandra Ruiz‐Alonso; Alaitz Zabala; Patricia Gálvez‐M...; Juan Antonio Marchal; Blanca Vázquez‐Lasa; Idoia Gallego; Laura Saenz‐del‐B...; Jose Luis Pedraz
• Keywords: 3D bioprinting; Biocompatibility; Chondrogenesis

Cartilage is a connective tissue which limited capacity for healing and repairing. In this context, osteoarthritis (OA) disease may be developed with high prevalence in the use of scaffolds promising treatment. addition, three-dimensional (3D) bioprinting has become an emerging additive manufacturing technology because its rapid prototyping possibility creating complex structures. This study focused on development nanocellulose-alginate (NC-Alg) based bioinks 3D cartilage regeneration to it added chondroitin sulfate (CS) dermatan (DS). First, rheological properties are evaluated. Then, sterilization effect, biocompatibility, printability NC-Alg-CS NC-Alg-DS inks Subsequently, printed characterized. Finally, loaded murine D1-MSCs-EPO cell viability functionality, as well chondrogenic differentiation ability assessed. Results show that addition both CS DS NC-Alg ink improves characteristics terms rheology functionality. Moreover, promoted scaffolds. Therefore, utilization MSCs containing feasible engineering approach regeneration.