Composite Hydrogel Containing Collagen‐Modified Polylactic Acid‐Hydroxylactic Acid Copolymer Microspheres Loaded with Tetramethylpyrazine Promotes Articular Cartilage Repair

• DOI: 10.1002/mabi.202400003
• Publication Date: 2024-04-10T09:26:30Z
• Authors: Yalan Pan; Bin Li; Xiaoxian Sun; Pengcheng Tu; Yang Guo; Zitong Zhao; Mao Wu; Yun Wang; Zhifang Wang; Yong Ma
• Keywords: Polylactic Acid; PLGA; Chondrogenesis; Articular cartilage repair; Biocompatibility

Articular cartilage defects pose a significant challenge due to the limited self-healing ability of cartilage. However, traditional techniques face limitations including autologous chondrocyte expansion issues. This study aims investigate effects polylactic acid-glycolic acid (PLGA) and collagen-surface modified (CPLGA) microspheres loaded with tetramethylpyrazine (TMP) on two cell types regeneration potential articular CPLGA are prepared by Steglich reaction characterized. They evaluated effect TMP-loaded HUVECs (Human Umbilical Vein Endothelial Cells) examined compatibility blank BMSCs (Bone marrow mesenchymal stromal cells) their promote differentiation. Subcutaneous implant immune tests defect treatment conducted assess biocompatibility repair potential. The results highlight efficacy in promoting tissue regeneration, attributed improved hydrophilicity collagen-induced mitigation degradation. Under hypoxic conditions, both PLGA exhibit inhibitory HUVEC proliferation, migration, angiogenesis. Notably, show enhanced BMSCs, facilitating chondrogenic Moreover, microsphere-composite hydrogel exhibits for modulating angiogenesis BMSC