Relationship between the Rod complex and peptidoglycan structure in Escherichia coli

• DOI: 10.1002/mbo3.1385
• Publication Date: 2023-10-12T01:01:53Z
• Authors: Risa Ago; Yuhei O. Tahara; Honoka Yamaguchi; Motoya Saito; Wakana Ito; Kaito Yamasaki; Taishi Kasai; Sho Okamoto; Taiki Chikada; Taku Oshima; Issey Osaka; Makoto Miyata; Hironori Niki; Daisuke Shiomi
• Keywords: Cytoskeletal Proteins; suppressor mutation; Cell Wall; Escherichia coli Proteins; Escherichia coli; Rod complex; Original Articles; Peptidoglycan; peptidoglycan; Microbiology; cell shape; QR1-502

Abstract Peptidoglycan for elongation in Escherichia coli is synthesized by the Rod complex, which includes RodZ. Although various mutant strains of complex have been isolated, relationship between activity and overall physical chemical structures peptidoglycan not reported. We constructed a RodZ mutant, termed RMR, analyzed growth rate, morphology, other characteristics cells producing complexes containing RMR. The morphology RMR were abnormal, we isolated suppressor mutants from cells. Most mutations found components suggesting that these increase integrity and/or complex. purified wild‐type, observed their detail. had many large holes different compositions muropeptides those WT may be determinant only whole shape but also its highly dense structure to support mechanical strength cell wall.