Using meta‐analysis to understand the impacts of dietary protein and fat content on the composition of fecal microbiota of domestic dogs (Canis lupus familiaris): A pilot study

• DOI: 10.1002/mbo3.1404
• Publication Date: 2024-03-22T05:50:00Z
• Authors: Francis D. Phimister; Rachel C. Anderson; David G. Thomas; Michelle J. Farquhar; Paul Maclean; Ruy Jauregui; Wayne Young; Christina F. Buto...; Emma N. Bermingham
• Keywords: crude protein; canine; microbiome; crude fat; Original Articles; Microbiology; QR1-502

Abstract The interplay between diet and fecal microbiota composition is garnering increased interest across various host species, including domestic dogs. While the influence of dietary macronutrients their associated microbial communities have been extensively reviewed, these reviews are descriptive do not account for differences in community analysis, nor they standardize macronutrient content studies. To address this, a meta‐analysis was performed to assess impact crude protein (“protein”) fat (“fat”) on healthy Sixteen publications met eligibility criteria meta‐analysis, yielding final data set 314 Diets were classed as low, moderate, high, or supra terms content. Sequence from each publication retrieved public databases reanalyzed using consistent bioinformatic pipelines. Analysis diversity indices unsupervised clustering with principal coordinate analysis revealed small effect size complete overlap levels at overall level. Supervised through random forest partial least squares‐discriminant indicated alterations more individual taxonomic level, corresponding fat. Prevotellaceae Ga6A1 group Enterococcus increasing protein, while Allobaculum Clostridium sensu stricto 13 Interestingly, analyses that Sharpea , despite its low relative abundance dog's microbiome, primarily responsible separation microbiome both Future research should focus validating understanding functional roles relatively low‐abundant genera.