Expression of receptors for advanced glycation end‐products (RAGE) is closely associated with the invasive and metastatic activity of gastric cancer

• DOI: 10.1002/path.1031
• Publication Date: 2002-10-06T11:25:48Z
• Authors: Hiroki Kuniyasu; Naohide Oue; Atsuko Wakikawa; Hideo Shigeishi; Norimasa Matsutani; Kazuya Kuraoka; Reiko Ito; Hiroshi Yokozaki; Wataru Yasui
• Keywords: RAGE; HMGB1

The receptor for advanced glycation end-products (RAGE) is a newly recognized factor regulating cancer cell invasion and metastasis. This study investigated the expression of RAGE in gastric carcinomas its association with Of eight lines examined, seven constitutively expressed messenger ribonucleic acid (mRNA), MKN45 being exception. protein MKN28 cells treated antisense S-oligodeoxynucleotide was nine times less than that sense S-oligodeoxynucleotide-treated cells. Growth under treatment not different from seen (a line producing high levels RAGE) non-RAGE-expressing line). suppressed invasive activity RAGE-positive cells, as estimated by vitro assay. number invading type IV collagen-coated membrane significantly lower (p<0.0001). In contrast, RAGE-negative showed no difference. A wound-healing assay migrated into scraped area, whereas many budding nests area. Immunohistochemistry carcinoma tissue 62 (65%) 96 cases examined were poorly differentiated adenocarcinomas preferentially (38/42, 90%) Strong immunoreactivity also correlated depth lymph node metastasis tended to be distributed at front primary tumours detected all metastatic foci nodes. major ligand, amphoterin, 82 (85%) cases, regardless histological disease progression. appears closely associated cancer.