Prognostic significance of Dicer expression in ovarian cancer—link to global microRNA changes and oestrogen receptor expression

• DOI: 10.1002/path.2658
• Publication Date: 2009-10-27T15:55:09Z
• Authors: Areeg Faggad; Jan Budczies; Oleg Tchernitsa; Silvia Darb‐Esfahani; Jalid Sehouli; Berit M Müller; Ralph Wirtz; Radoslav Chekerov; Wilko Weichert; Bruno Sinn; Christin Mucha; Nasr E Elwali; Reinhold Schäfer; Manfred Dietel; Carsten Denkert
• Keywords: Dicer; Drosha; Ribonuclease III

MicroRNA (miRNA) deregulation is a hallmark of human cancer. However, the mechanisms underlying miRNA alteration and specific role proteins involved in processing remains to be elucidated. Dicer key enzyme pathway that essential for production mature miRNAs from their precursors. We tested hypothesis has biological clinical relevance ovarian cancer, using range methods including vitro manipulation expression. observed down-regulation subgroup carcinomas, found decreased expression correlates significantly with reduced patient survival serous cancers advanced disease stages. Moreover, microarray functional analysis suggest connected global microRNAome gene changes, particularly oestrogen receptor (ER) mRNA protein tumour tissue cell culture. Our data common mechanism changes by alterations basic machinery controlling biogenesis, which central enzyme. These are prognostic value may play molecular pathogenesis carcinoma and, possibly, other tumours. Knowledge these pathways help toward new targeted therapeutic approaches