Molecular subtyping reveals immune alterations in IDH wild‐type lower‐grade diffuse glioma

• DOI: 10.1002/path.5468
• Publication Date: 2020-05-17T16:10:17Z
• Authors: Fan Wu; Guan‐Zhang Li; Han‐Jie Liu; Zheng Zhao; Rui‐Chao Chai; Yu‐Qing Liu; Hao‐Yu Jiang; You Zhai; Yue‐Mei Feng; Ren‐Peng Li; Wei Zhang
• Keywords: Subtyping; Immune escape

Isocitrate dehydrogenase (IDH) wild-type diffuse lower-grade glioma (LGG) is usually associated with poor outcome, but there have been disputes over its clinical outcome and classification. We present here a robust gene expression-based molecular classification of IDH LGG into two subtypes distinct biological features. A discovery cohort 49 LGGs from the Chinese Glioma Genome Atlas (CGGA) was subjected to clustering function analysis. Seventy-three tumors The Cancer (TCGA) were used validate our findings. Consensus transcriptional data uncovered concordant prognostically significant LGG. Subtype 1, poorer outcomes, characterized by significantly higher immune cytolytic scores, M2 macrophages, up-regulation exhaustion markers, while 2, which had elevated lymphocytes plasma cells, showed relatively favorable survival. Somatic alteration analysis revealed that 1 more frequently deleted regions, such as locus CDKN2A/CDKN2B, DMRTA1, C9orf53, MTAP. Furthermore, we developed validated five-gene signature for better application this acquired stratification. Our demonstrate prognostic heterogeneity within deepen understandi-g tumor entity. © 2020 Pathological Society Great Britain Ireland. Published John Wiley & Sons, Ltd.