Unique growth pattern of human mammary epithelial cells induced by polymeric nanoparticles

• DOI: 10.1002/phy2.27
• Publication Date: 2013-09-17T03:59:27Z
• Authors: Rajaa Hussien; Bertrand H. Rihn; Housam Eidi; Carole Ronzani; Olivier Joubert; Luc Ferrari; Oscar Vazquez; Daniela Kaufer; George A. Brooks
• Keywords: Physiology; 1.1 Normal biological development and functioning; [SDV]Life Sciences [q-bio]; Medical Biotechnology; Clinical Sciences; Medical Physiology; 610; Bioengineering; Eudragit® RS nanoparticles; Cell growth; 03 medical and health sciences; Underpinning research; Medical physiology; Breast Cancer; Nanotechnology; Eudragit((R)) RS nanoparticles; Cancer; Original Research; fetal bovine serum; 0303 health sciences; Biomedical and Clinical Sciences; epithelial cells; 3. Good health; cell proliferation; nanoparticles; Generic health relevance; Biotechnology

Due to their unique properties, engineered nanoparticles (NPs) have found broad use in industry, technology, and medicine, including as a vehicle for drug delivery. However, the understanding of NPs’ interaction with different types mammalian cells lags significantly behind increasing adoption In this study, we show responses human epithelial breast when exposed polymeric Eudragit® RS NPs (ENPs) 1–3 days. Cells displayed dose-dependent increases metabolic activity growth, but lower proliferation rates, than control cells, evidenced tetrazolium salt (WST-1) 5-bromo-2′-deoxyuridine (BrdU) assays, respectively. Those effects did not affect cell death or mitochondrial fragmentation. We attribute increase growth culture ENPs three factors: (1) high affinity proteins present serum ENPs, (2) adhesion (3) activation pathways. The genes responsible stimulating were identified by mass spectrometry Microarray analyses. demonstrate novel property which act organize epithelium determined analysis.