Development of a novel vector for siRNA delivery based on arginine‐modified polyvinylamine

• DOI: 10.1002/pi.6386
• Publication Date: 2022-03-03T13:05:25Z
• Authors: Mei Lin; Aizhu Lin; Sheng Huang; Tianhui Liu; Fang Ke; Deshun Qiu; Xuefeng Lin; Daoshu Luo
• Keywords: Zeta potential

Abstract An ideal carrier that delivers small interfering RNA (siRNA) should be designed based on two criteria: high gene transfection efficiency and low cytotoxicity. In this work, arginine was used to modify polyvinylamine (PVAm) form a novel PVAm derivative (PVAm‐Arg) for siRNA delivery the characterized using Fourier transform infrared spectroscopy. PVAm‐Arg/siRNA complexes were formed by self‐assembly method. The zeta potential size of measured electrophoretic light scattering dynamic scattering, respectively. binding capacity PVAm‐Arg assessed gel retardation assay. results showed derivatives can bind effectively with sizes about 72 ± 2 nm. What is more, ability condense better than PVAm. Cytotoxicity assay RSC96 cells also had lower cytotoxicity compared in vitro cellular uptake assay, good efficiency. Therefore, we concluded would promising candidate as vector. © 2022 Society Industrial Chemistry.