Profiling of the human monocytic cell secretome by quantitative label‐free mass spectrometry identifies stimulus‐specific cytokines and proinflammatory proteins

• DOI: 10.1002/pmic.201200108
• Publication Date: 2012-07-26T14:51:58Z
• Authors: M. Groessl; H. Luksch; A. Rösen‐Wolff; A. Shevchenko; M. Gentzel
• Keywords: Proinflammatory cytokine

The immune response to pathogens or injury relies on the concerted release of cytokines and proteins with biological activity important for host protection, defense, wound healing. Consequently, secretome cells provides a promising resource discovery specific molecular markers targets pharmacological intervention. Here, we employ label-free MS unbiased, quantitative profiling human monocytic cell under different proinflammatory stimuli. profiles reveal highly stimulus-dependent cellular differential, secretion more than 200 proteins, including cytokines.