Eye proteome of Drosophila melanogaster

• DOI: 10.1002/pmic.202300330
• Publication Date: 2023-11-15T03:31:46Z
• Authors: Mukesh Kumar; Canan Has; Khanh Lam‐Kamath; Sophie Ayciriex; Deepshe Dewett; Mhamed Bashir; Clara Poupault; Kai Schuhmann; Henrik Thomas; Oskar Knittelfelder; Bharath Kumar Rag...; Robert Ahrends; Jens Rister; Andrej Shevchenko
• Keywords: Male; Proteomics; 106037 Proteomik; Proteome; 301306 Medizinische Molekularbiologie; 106037 Proteomics; 301306 Medical molecular biology; absolute quantification; phototransduction; Eye; eye proteome; Drosophila melanogaster; Tandem Mass Spectrometry; Animals; Drosophila Proteins; Eye Proteins

AbstractDrosophila melanogaster is a popular model organism to elucidate the molecular mechanisms that underlie the structure and function of the eye as well as the causes of retinopathies, aging, light‐induced damage, or dietary deficiencies. Large‐scale screens have isolated genes whose mutation causes morphological and functional ocular defects, which led to the discovery of key components of the phototransduction cascade. However, the proteome of the Drosophila eye is poorly characterized. Here, we used GeLC‐MS/MS to quantify 3516 proteins, including the absolute (molar) quantities of 43 proteins in the eye of adult male Drosophila reared on standard laboratory food. This work provides a generic and expandable resource for further genetic, pharmacological, and dietary studies.