Rational peptide design for targeting cancer cell invasion

• DOI: 10.1002/prot.26580
• Publication Date: 2023-08-30T12:15:27Z
• Authors: Mohammad Mahmoudi...; Seyed Shahriar Arab; Saeed Balalaie; Sorour Ramezanpour; Arshad Hosseini; Nikolay V. Dokholyan; Parastoo Tarighi
• Keywords: Humans; Neoplasm Invasiveness; Peptides; Urokinase-Type Plasminogen Activator; 3. Good health; Receptors, Urokinase Plasminogen Activator

Abstract Cell invasion is an important process in cancer progression and recurrence. Invasion implantation of cells from their original place to other tissues, by disabling vital organs, challenges the treatment patients. Given importance matter, many molecular treatments have been developed inhibit cell invasion. Because low production cost ease production, peptides are valuable therapeutic molecules for inhibiting In recent years, advances field computational biology facilitated design anti‐cancer peptides. our investigation, using approaches such as evolutionary analysis, residue scanning, protein–peptide interaction dynamics, free energy team designed a peptide library with about 100 000 candidates based on A6 (acetyl‐KPSSPPEE‐amino) sequence which anti‐invasion peptide. During studies, two that give highest scores showed greatest similarity were entered into experimental analysis workflow further analysis. steps, anti‐metastatic potency effects evaluated MTT assay, RT‐qPCR, zymography assay. Our study disclosed IK1 (acetyl‐RPSFPPEE‐amino) peptide, like A6, has great cells.