Berberine ameliorates lipopolysaccharide‐induced acute lung injury via the PERK‐mediated Nrf2/HO‐1 signaling axis

• DOI: 10.1002/ptr.6206
• Publication Date: 2018-10-22T09:29:49Z
• Authors: Yuan Liang; Chongxi Fan; Xiaolong Yan; Xi Lu; Hua Jiang; Shouyin Di; Zhiqiang Ma; Yingtong Feng; Zhengbin Zhang; Pan Feng; Xiao Feng; Jianyu Feng; Faguang Jin
• Keywords: Lipopolysaccharides; Male; 0301 basic medicine; 03 medical and health sciences; Berberine; NF-E2-Related Factor 2; Acute Lung Injury; Animals; Humans; Heme Oxygenase-1; Signal Transduction; 3. Good health

A fundamental element of acute lung injury (ALI) is the inflammatory response, which can affect entire respiratory system, including tract and alveoli. Berberine has gained attention because its anti‐inflammatory effects. Nuclear factor‐erythroid 2‐related factor 2 (Nrf2) endoplasmic reticulum (ER) stress are involved in injury. Nrf2 also acts as a protein kinase‐like ER kinase (PERK) substrate heart disease. Therefore, this study investigated effect berberine against lipopolysaccharide (LPS)‐induced ALI role PERK‐mediated Nrf2/HO‐1 signaling axis. promoted nuclear translocation phosphorylation vitro. After LPS stimulation, was further enhanced, whereas (IL‐6 IL‐8) release reactive oxygen species generation were significantly decreased. effectively alleviated by reducing edema neutrophil infiltration. reduced histopathological changes via inhibition activation signaling. Thapsigargin‐induced small interference RNA (siRNA)‐mediated abrogated protective effects vitro, siRNA‐mediated suppression sulforaphane‐induced improved those Importantly, induction led to activation, PERK depletion partly level LPS‐induced cells. inhibits through