α‐Asarone attenuates chronic sciatica by inhibiting peripheral sensitization and promoting neural repair

• DOI: 10.1002/ptr.7603
• Publication Date: 2022-09-08T00:44:12Z
• Authors: Di Zhang; Xin Li; Bei Jing; Zhenni Chen; Huimei Shi; Yachun Zheng; Shiquan Chang; Jianxin Sun; Guoping Zhao
• Keywords: Dorsal root ganglion; TRPM8

This study explored the therapeutic effect of α-asarone on chronic sciatica. Thirty-two Sprague-Dawley (SD) rats were divided into four groups: sham group, constriction injury (CCI) pregabalin and group. Hot hyperalgesia was induced after CCI operation, found to relieve neuralgia. Furthermore, reduced IL1β, IL6, TNF-α, CRP, LPS levels increased IL10 in serum. α-Asarone decreased protein TRPA1, TRPM8, TRPV1-4 mRNA TRPV1-4, TNF-α dorsal root ganglion neurons. In sciatic nerve, treatment number inflammatory cells promoted proliferation Schwann cells, favouring recovery nerve structure. cellular experiments, cell apoptosis via TLR4/p38MAPK signalling; attenuated LPS-induced by decreasing TLR4, p-p38MAPK, cleaved-caspase3, cleaved-caspase7 increasing Bcl-2 Bcl-xl expression. Overall, these findings suggest that relieves sciatica factors, inhibiting peripheral sensitization, repair damaged nerves.