Quercetin Alleviates Chronic Urticaria by Negatively Regulating IgE‐Mediated Mast Cell Activation Through CD300f

• DOI: 10.1002/ptr.8516
• Publication Date: 2025-05-01T11:41:18Z
• Authors: Chenrui Zhao; Na Wang; Chao Wang; Yujuan Yuan; Hongfen Du; Yuanyuan Ding; Hongli An

Chronic urticaria (CU) is a skin allergy caused by the excessive activation of mast cells. The main etiology CU type I allergic reaction mediated immunoglobulin (Ig)E. This study mainly explored therapeutic effect quercetin in ovalbumin (OVA)-induced mice and investigated its target mechanism vitro. symptom-alleviating was assessed model. possible molecular mechanisms were initially inferred through bioinformatic multi-database analyses. Quercetin targets examined using cell experiments with CD300f knockdown. RT-PCR western blot performed to verify quercetin. relieved wheal scratching times on back as well reduced eosinophilic infiltration degranulation lesions inhibited release IgE, histamine, TNF-α, MCP-1, IL-13 serum mice. In addition, it exhibited potential effects PI3K-Akt signaling pathway. Meanwhile, upregulated CU, activated CD300f, induced downstream SHP-1 phosphorylation. Of note, bound prevent IgE-mediated LAD2 β-hexosaminidase release, histamine Ca2+ influx, degranulation, F-actin cytoskeleton remodeling inhibiting AKT/IKK/NF-κB inflammatory results suggest that alleviates activating CD300f/SHP-1 activates inhibit