Hematopoietic Function Restoration by Transplanting Bone Marrow Niches In Vivo Engineered Using Carbonate Apatite Honeycomb Bioreactors

• DOI: 10.1002/sstr.202400065
• Publication Date: 2024-06-19T17:53:54Z
• Authors: Koichiro Hayashi; Ryo Kishida; Akira Tsuchiya; Kunio Ishikawa
• Keywords: Hematopoietic stem cell

Hematopoietic stem cell (HSC) transplantation is used to treat blood and immunodeficient diseases. HSC expansion techniques must be developed prevent complications ensure reliable therapeutic efficacy. Hence, several studies have attempted in vitro of HSCs using scaffolds but failed mimic the diverse complex nature environments. Herein, an artificial microenvironment, bone marrow (BM) niches created, through vivo engineering carbonate apatite honeycomb potential these restoring lost hematopoietic function immunity investigated. BM are generated every channel, wherein gradually aggregated. Compared actual BM, exhibit a 9.9‐ 78‐fold increase number stored CD45 − CD34 + side scatter low cells that mainly considered at 8 12 weeks, respectively. The scaffold containing into immunocompromised mice increases peripheral chimerism restores immunocytes (monocytes lymphocytes) normal levels. This study contributes development efficient techniques. Additionally, vivo‐engineered integrated tissues can elucidate interplay between resident cells.