Correlations Between the Levels of Oct4 and Nanog as a Signature for Naïve Pluripotency in Mouse Embryonic Stem Cells

• DOI: 10.1002/stem.1230
• Publication Date: 2012-09-12T01:42:51Z
• Authors: Silvia Muñoz Desc...; Pau RuÉ; Jordi Garcia‐Ojalvo; Alfonso Martinez ...
• Keywords: Homeobox protein NANOG; Rex1

The pluripotent state is traditionally associated with large absolute levels of certain transcription factors such as Nanog and Oct4. Here, we present experimental observations using quantitative immunofluorescence that pluripotency in mouse embryonic stem cells (mESCs) established by specific ratios between Oct4 Nanog. When are grown 2i conditions, they exhibit uniform this a high correlation the individual cells. lost when differentiate. Our results suggest these two distribution Oct4/Nanog can be used quantifiers to distinguish three subpopulations an mESC culture: pluripotent, lineage-primed, differentiating apply lower or mutant for β-Catenin Tcf3, higher probability differentiation.