Human umbilical cord-derived mesenchymal stem cells elicit macrophages into an anti-inflammatory phenotype to alleviate insulin resistance in type 2 diabetic rats

• DOI: 10.1002/stem.2238
• Publication Date: 2015-11-02T23:16:45Z
• Authors: Zongyan Xie; Haojie Hao; Chuan Tong; Yu Cheng; Jiejie Liu; Yaping Pang; Yiling Si; Yulin Guo; Li Zang; Yiming Mu; Weidong Han
• Keywords: Macrophage polarization; M2 Macrophage; Adipose tissue macrophages

Insulin resistance, a major characteristic of type 2 diabetes (T2D), is closely associated with adipose tissue macrophages (ATMs) that induce chronic low-grade inflammation. Recently, mesenchymal stem cells (MSCs) have been identified in alleviation insulin resistance. However, the underlying mechanism still remains elusive. Thus, we aimed to investigate whether effect MSCs on resistance was related phenotypes tissues T2D rats. In this study, human umbilical cord-derived (UC-MSCs) infusion produced significantly anti-diabetic effects and promoted sensitivity rats were induced by high-fat diet combined streptozotocin directed ATMs into an alternatively activated phenotype (M2, anti-inflammatory). vitro, MSC-induced M2 alleviated caused classically (M1, pro-inflammatory). Further analysis showed M1 stimulated UC-MSCs increase expression interleukin (IL)-6, molecule which upregulated IL4R expression, phosphorylation STAT6 macrophages, eventually polarized phenotype. Moreover, largely abrogated small interfering RNA (siRNA) knockdown IL-6. Together, our results indicate can alleviate part via production IL-6 elicits polarization. Additionally, obesity increased pro-inflammatory infiltration. may be new treatment for obesity-related concerning macrophage effects.