H3K4 Methyltransferase Set1a Is A Key Oct4 Coactivator Essential for Generation of Oct4 Positive Inner Cell Mass

• DOI: 10.1002/stem.2250
• Publication Date: 2016-01-19T17:17:28Z
• Authors: Lan Fang; Jun Zhang; Hui Zhang; Xiaoqin Yang; Xueling Jin; Ling Zhang; David G. Skalnik; Ying Jin; Yong Zhang; Xingxu Huang; Jiwen Li; Jiemin Wong
• Keywords: KLF4; Chromatin immunoprecipitation

Abstract Limited core transcription factors and transcriptional cofactors have been shown to govern embryonic stem cell (ESC) circuitry pluripotency, but the molecular interactions between remains ill defined. Here, we analyzed protein–protein Oct4, Sox2, Klf4, Myc (abbreviated as OSKM) a large panel of cofactors. The data reveal both specific common OSKM We found that among SET1/MLL family H3K4 methyltransferases, Set1a specifically interacts with Oct4 this interaction is independent Wdr5. recruited required for methylation at target gene promoters activation genes in ESCs, consistently ESC maintenance induced pluripotent generation. Gene expression profiling chromatin immunoprecipitation-seq analyses demonstrate broad involvement strong enrichment TSS sites. knockout study demonstrates not only mouse early development also generation Oct4-positive inner mass. Together our provides valuable information on mechanisms functional importance ESCs development.