Effect of particulate matter 2.5 on gene expression profile and cell signaling in JEG‐3 human placenta cells

• DOI: 10.1002/tox.22591
• Publication Date: 2018-07-05T10:12:13Z
• Authors: Woong Kim; Yoon Cho; Mi‐Kyung Song; Jung‐hee Lim; Jin young Kim; Myung Chan Gye; Jae‐Chun Ryu
• Keywords: KEGG; Toxicant

Particulate matter the environmental toxicant, with a diameter less than or equal to 2.5 μm (PM2.5 ) is common cause of several respiratory diseases. In recent years, studies have suggested that PM2.5 can influence diverse diseases, such as cardiovascular metabolic dementia, and female reproductive disorders, unhealthy birth outcomes. addition, epidemiological reported adverse health effects differ depending on regional variations. present study, evaluate specific , we collected two different samples from an underground parking lot ambient air, evaluated cytotoxicity eight cell lines originating human organs. Then, selected JEG-3 placenta cells, which show high both PM samples. Through RNA sequencing, gene expression profiling, ontology (GO) analysis after exposure samples, identified 1021 commonly expressed genes involved in immune responses, regulation apoptosis, so forth, are known induce effects. related calcium-signaling pathway, steroid hormone biosynthesis, cytokine-cytokine receptor interaction through Kyoto Encyclopedia Genes Genomes (KEGG) analysis. confirmed these expressions using qRT-PCR, protein levels mitogen-activated kinases COX-2 progesterone decreased western blotting enzyme-linked immunosorbent assay. conclusion, this study suggests possible toxic mechanism might be associated -induced disorders.