Effects of environmental chemicals on the proliferation and differentiation of neural stem cells

• DOI: 10.1002/tox.22829
• Publication Date: 2019-08-10T06:24:29Z
• Authors: Min Huang; Yingying Li; Kexin Wu; Shaowen Hao; Qian Cai; Zhi‐jun Zhou; Hui‐fang Yang
• Keywords: Paraquat; 0303 health sciences; PAX6 Transcription Factor; Apoptosis; Cell Differentiation; Methylmercury Compounds; Fetal Blood; 03 medical and health sciences; Gene Expression Regulation; Neural Stem Cells; Animals; Humans; Environmental Pollutants; Octamer Transcription Factor-3; Cells, Cultured; Cell Proliferation

Abstract The objective of the present study was to explore effects environmental chemicals, such as methyl mercury, paraquat, and bisphenol A, on cell proliferation apoptosis, well expression levels neuronal differentiation‐related genes in neural stem cells (NSCs). NSCs originated from human umbilical cord blood (HUCB‐NSCs) were used models current study. CCK‐8 flow cytometry experiments performed assess A apoptosis HUCB‐NSCs at different processes, including differentiation stages. expressions determined by reverse transcription‐polymerase chain reaction western blot analysis. results showed that treatments significantly inhibited induced HUCB‐NSCs, decreased Oct4, Gdf3, Sox1, whereas increased Pax6 Ngn1 both mRNA protein levels. In conclusion, this demonstrates chemicals can impair NSCs, which may cause abnormal development nervous system.