Ginsenoside Rb1 protects porcine oocytes against methylglyoxal damage thus it improves the quality of parthenogenetic activation and in vitro fertilization embryos

• DOI: 10.1002/tox.23063
• Publication Date: 2020-11-25T10:57:29Z
• Authors: Zhao‐Bo Luo; Mei‐Fu Xuan; Sheng‐Zhong Han; Zhou‐Yan Li; Nasar Khan; Biao‐Hu Quan; Xi‐Jun Yin; Jin‐Dan Kang
• Keywords: 0301 basic medicine; Ginsenosides; Swine; Parthenogenesis; Embryonic Development; Panax; Fertilization in Vitro; Pyruvaldehyde; Antioxidants; In Vitro Oocyte Maturation Techniques; 3. Good health; 03 medical and health sciences; Blastocyst; Oogenesis; Oocytes; Animals; Female; Reactive Oxygen Species; DNA Damage

AbstractPanax ginseng, a functional food, has been widely used as an edible nourishment and medicinal supplement. Ginsenoside Rb1 is a major bioactive ingredient of ginseng, which shows very specific anti‐apoptosis and anti‐oxidant activities. Methylglyoxal (MGO) is one of intermediate products of glucose metabolism, which is absorbed easily from high sugar foods or carbonated beverages. It may involve in a variety of detrimental processes in vivo. However, it has not been fully explored the effects of ginsenoside Rb1 on MGO‐induced oocytes damage. This study found that MGO‐induced DNA damage and mitochondrial dysfunction result in the failure of porcine oocytes maturation and low in vitro development capacity of parthenogenetic activation (PA) and in vitro fertilization (IVF) embryos. Conversely, Rb1 supplementation recovered the rate of maturation, and improved in vitro development capacity of PA and IVF embryos. Rb1 also provided porcine oocytes a lower level of reactive oxygen species production, higher level of ATP content and mitochondrial membrane potential, and stimulated pluripotency gene expression in blastocysts. The findings of this study reveal ginsenoside Rb1 protects porcine oocyte from the cytotoxicity effects of methylglyoxal and provides novel perspectives for the protection of reproduction system by functional food of ginseng.