SRBD1 Regulates the Cell Cycle, Apoptosis, and M2 Macrophage Polarization via the RPL11‐MDM2‐p53 Pathway in Glioma

• DOI: 10.1002/tox.24396
• Publication Date: 2024-09-11T09:32:50Z
• Authors: Hongfu Chen; Shuping Gao; Peng Wang; Manyi Xie; Hui Zhang; Yuechao Fan; Er Nie; Qing Lan
• Keywords: Immunoprecipitation

ABSTRACT Low expression of certain ribosomal proteins leads to the inactivation p53, which is mediated mainly by RPL5 or RPL11 (ribosomal protein L11). It also unknown what mechanisms drive aberrant in tumor. SRBD1 (S1 RNA‐binding domain 1), as a highly conserved protein, lowly expressed glioma tissues and correlated with prognosis. In this study, we observed that was closely related p53 signaling. The upregulation elevated levels, thereby activating signaling pathway. As an RNA bind could 5′‐UTR target genes regulate translation. We further conducted immunoprecipitation using anti‐SRDB1 antibody noticed 29 hub RNA, including RPL11. inhibit MDM2‐mediated ubiquitination. promoted binding MDM2 via elevating turn activated Disrupting blocked SRBD1‐induced suppression. mouse xenograft model, ectopic effective reducing total M2 tumor‐associated macrophages (TAMs) density suppressed tumor growth. summary, these data show has critical role inhibition growth macrophage polarization, targeting RPL11‐MDM2‐p53 may be strategy improve therapy survival for patients.