Mastitis on selected farms in Wakiso district, Uganda: Burden, pathogens and predictors of infectivity of antimicrobial resistant bacteria in dairy herds

• DOI: 10.1002/vms3.1234
• Publication Date: 2023-08-21T16:30:28Z
• Authors: Steven Kakooza; Francis Mutebi; Paul Ssajjakambwe; Eddie Wampande; Esther Nabatta; Collins Atuheire; Sayaka Tsuchida; Torahiko Okubo; Kazunari Ushida; John Baligwamunsi...
• Keywords: Milking; California mastitis test

Abstract Background Mastitis and associated antimicrobial resistance (AMR) are major challenges to the dairy industry worldwide. Objective This study aimed expose mastitis burden, causative bacteria drivers for mastitis‐causing multi‐drug‐resistant (MDR) Staphylococci infectivity in cows on farms Wakiso district, Uganda. Methods On 22 farms, practices were documented using questionnaires, 175 screened by California test. Composite milk samples from positive reactors submitted laboratory bacterial culture testing. Antimicrobial sensitivity testing Kirby Bauer disc diffusion method was done only with a panel of 10 antimicrobials clinical relevance. Results detected 80.6% ( n = 141) sampled cows, which sub‐clinical (76.0%: 133) predominant. The Chi‐squared analysis hypothesized that cow age p 0.017), sub‐county 0.013), parity < 0.0001), sex farm owner 0.003), duration production 0.048) use milking salve 0.006) mastitis. Coagulase‐negative most prevalent (71.4%; 95), followed Staphylococcus aureus (30.1%, 40). (76.3%; 135) majorly resistant penicillin tetracycline. Only one isolate phenotyped as methicillin‐resistant specie (MRSS). prevalences MDR strains at level 6.3% 8.3%. phenotype identified penicillin–tetracycline–trimethoprim‐sulphamethoxazole. an MRSS exhibited broadest pattern. Cow predictor herds. Conclusion high prevalence pathogen AMR found exposes possibilities economic losses sector warranting need farmer sensitization institution proper prevention control programs, emphasis hygiene routine disease monitoring.