IRES-Dependent Second Gene Expression Is Significantly Lower Than Cap-Dependent First Gene Expression in a Bicistronic Vector

• DOI: 10.1006/mthe.2000.0050
• Publication Date: 2002-09-18T19:46:43Z
• Authors: Hiroyuki Mizuguchi; Zhili Xu; Akiko Ishii-Watabe; Eriko Uchida; Takao Hayakawa
• Keywords: Heterologous expression

The internal ribosome entry site (IRES) has been widely used to coexpress heterologous gene products by a message from single promoter. However, little is known about the efficiency of IRES-dependent second expression in comparison with that first expression. This study was undertaken characterize relative bicistronic vector, which derived 5′ untranslated regions encephalomyocarditis virus (EMCV). compared cap-dependent several cultured cell lines and mouse liver vivo. ranged 6 100% (in most cases between 20 50%) gene. Second plasmid without IRES 0.1–0.8% (with some exceptions) These findings have important implications for use IRES, i.e., care should be taken regarding decreased capacity downstream as well determining positioned or vector.