Monitoring of Atg5-Independent Mitophagy

• DOI: 10.1007/7651_2017_16
• Publication Date: 2017-04-29T10:03:45Z
• Authors: Satoko Arakawa; Shinya Honda; Satoru Torii; Masatsune Tsujioka; Shigeomi Shimizu
• Keywords: Erythrocytes; Mitophagy; Flow Cytometry; Autophagy-Related Protein 5; Cell Line; Mitochondria; Gene Knockout Techniques; Mice; Genes, Reporter; Animals; Humans; Biological Assay; Sequence Deletion; Signal Transduction

Mitophagy is a mitochondrial quality control mechanism where damaged and surplus mitochondria are removed by autophagy. There are at least two different mammalian autophagy pathways: the Atg5-dependent conventional pathway and an Atg5-independent alternative pathway; the latter is involved in the erythrocyte mitophagy. In this chapter we describe the various experimental approaches to assess Atg5-indepedndent mitophagy in mammalian cells.