In Vitro 3D Mechanical Stimulation to Tendon-Derived Stem Cells by Bioreactor

• DOI: 10.1007/7651_2021_432
• Publication Date: 2022-01-14T09:50:22Z
• Authors: Ziming Chen; Peilin Chen; Rui Ruan; Minghao Zheng
• Keywords: mechanobiology

Bioreactors can offer an advanced platform to provide conditions that mimic the native microenvironment, which can also provide stretching environment for mechanobiology research. Tendon-derived stem cells (TDSCs) are a type of mechanosensitive and multipotent cells, which behave differently in diverse mechanical stretching environments. We have proved the in vitro three-dimensional (3D) mechanical stimulation could closely mimic the stretching environment in vivo. Thus, here we describe applying a customized bioreactor to provide 3D force for mechanical stimulation on TDSC in vitro.