Efficient Transduction of Dendritic Cells Using Coronavirus-Based Vectors

• DOI: 10.1007/978-0-387-33012-9_35
• Publication Date: 2007-04-30T23:59:49Z
• Authors: Klara K. Eriksson; Divine Makia; Reinhard Maier; Luisa Cervantes; Burkhard Ludewig; Volker Thiel
• Keywords: DNA, Complementary; Models, Genetic; Genetic Vectors; Dendritic Cells; Models, Biological; Article; Cell Line; 3. Good health; Coronavirus; Mice; Genetic Techniques; Genes, Reporter; Animals; RNA, Viral; RNA, Messenger; Plasmids

To investigate to what extent coronavirus vectors can induce antitumoral and antiviral humoral and cellular immune responses in vivo, we generated vectors based on mouse hepatitis virus to be used for studies of the immunological response to antigens expressed on murine dendritic cells. In these vectors, the structural genes encoding the viral envelope (E) and membrane (M) proteins have been deleted and replaced by sequences encoding a reporter protein (green fluorescent protein [GFP]) fused to an