Selenium as a Versatile Center in Fluorescence Probe for the Redox Cycle Between HClO Oxidative Stress and H2S Repair

• DOI: 10.1007/978-1-4939-1441-8_8
• Publication Date: 2014-10-16T11:56:50Z
• Authors: Zhangrong Lou; Peng Li; Keli Han
• Keywords: Magnetic Resonance Spectroscopy; Microscopy, Confocal; Time Factors; Hydrogen-Ion Concentration; Cell Line; Hypochlorous Acid; 3. Good health; Mice; Oxidative Stress; Selenium; Spectrometry, Fluorescence; Absorption, Physicochemical; Reducing Agents; Benzene Derivatives; Animals; Humans; Hydrogen Sulfide; Reactive Oxygen Species; Acids; Oxidation-Reduction; Fluorescent Dyes

Selenium is a biologically important trace element and acts as an active center of glutathione peroxidase (GPx). GPx is the important antioxidant enzyme to protect organisms from oxidative damage via catalyzing the reaction between ROS and glutathione (GSH). Mimicking the oxidation-reduction cycles of the versatile selenium core in GPx, we can develop fluorescence probes to detect oxidation and reduction events in living systems. The cellular redox balance between hypochloric acid (HClO) and hydrogen sulfide (H2S) has broad implications in human health and diseases, such as Alzheimer's disease (AD). Therefore, to further investigate the roles of this redox balance and understand the pathogenesis of neurodegenerative diseases, it is necessary to detect the redox state between HClO and H2S in real time. We have developed a reversible fluorescence probe MPhSe-BOD for imaging of the redox cycle between HClO and H2S based on oxidation and reduction of selenide in living cells.