Clonetegration Using OSIP Plasmids: One-Step DNA Assembly and Site-Specific Genomic Integration in Bacteria

• DOI: 10.1007/978-1-4939-6343-0_11
• Publication Date: 2016-09-26T11:24:58Z
• Authors: Lun Cui; Keith E. Shearwin
• Keywords: DNA, Bacterial; Mutagenesis, Insertional; Viral Proteins; Base Sequence; Integrases; Escherichia coli; Bacteriophages; Transformation, Bacterial; Cloning, Molecular; Genetic Engineering; Genome, Bacterial; Plasmids

Clonetegration is a method for site-specific insertion of DNA into prokaryotic chromosomes, based on bacteriophage integrases. The method combines DNA cloning/assembly and chromosomal integration into a single step, providing a simple and rapid strategy for inserting DNA sequences into bacterial chromosomes.