Quantitative Analysis of the Rates for Repeat-Mediated Genome Instability in a Yeast Experimental System

• DOI: 10.1007/978-1-4939-7306-4_29
• Publication Date: 2017-10-16T16:23:08Z
• Authors: Elina A. Radchenko; Ryan J. McGinty; Anna Y. Aksenova; Alexander J. Neil; Sergei M. Mirkin
• Keywords: Repeated sequence; Chromosome instability

Instability of repetitive DNA sequences causes numerous hereditary disorders in humans, the majority of which are associated with trinucleotide repeat expansions. Here, we describe a unique system to study instability of triplet repeats in a yeast experimental setting. Using fluctuation assay and the novel program FluCalc we are able to accurately estimate the rates of large-scale expansions, as well as repeat-mediated mutagenesis and gross chromosomal rearrangements for different repeat sequences.