GapmeR-Mediated Gene Silencing in Motile T-Cells

• DOI: 10.1007/978-1-4939-9036-8_9
• Publication Date: 2019-01-04T16:43:21Z
• Authors: Mobashar Hussain ...; Seow Theng Ong; Madhavi Latha Som...; Atish Kizhakeyil; Navin Kumar Verma
• Keywords: Cell Movement; Gene Knockdown Techniques; T-Lymphocytes; Genetic Vectors; Humans; Gene Silencing; Oligonucleotides, Antisense; Transfection; Cells, Cultured; 3. Good health

Gene silencing is an important method to study gene functions in health and diseases. While there are various techniques that are applied to knockdown specific gene(s) of interest, they have certain limitations in application to T-lymphocytes. T-cells are "hard-to-transfect" cells and are recalcitrant to transfection reagents. Here, we describe the use of novel cell-permeating antisense molecules, called "GapmeR", to knockdown specific gene(s) in human primary T-cells.