Mass Spectrometry Applied to Human Cerebrospinal Fluid Lipidome

• DOI: 10.1007/978-1-4939-9706-0_23
• Publication Date: 2019-08-20T20:05:33Z
• Authors: Laura Millán; Joaquín Fernández...; Enrique Santamaría; Rebeca Mayo
• Keywords: Lipidome

Lipidomics aims at characterizing lipid profiles and their biological role with respect to protein expression involved in lipid metabolism. Specifically, cerebrospinal fluid (CSF) lipidomics is offering a new perspective in the search for surrogate biomarkers to facilitate early diagnosis of psychiatric and neurodegenerative diseases. In this chapter, we describe a nontargeted approach to profile lipid molecular species present in human CSF using ultrahigh-performance liquid chromatography-electrospray ionization-time-of-flight mass spectrometry (UPLC-ESI-ToF-MS). This workflow complements the toolbox useful for the exploration and monitoring neurodegenerative mechanisms associated with a dysregulation in lipid metabolism.