Modified carbon flux during oxygen limited growth of Corynebacterium glutamicum and the consequences for amino acid overproduction

• DOI: 10.1007/bf00129316
• Publication Date: 2004-11-02T13:55:30Z
• Authors: H Dominguez; C Nezondet; N D Lindley; M Cocaign
• Keywords: 0301 basic medicine; 03 medical and health sciences

The amino acid producing bacterium Corynebacterium glutamicum accumulated lactate, succinate and acetate under oxygen-limited growth conditions. Significant restructuring of carbon flux through the central metabolic pathways occurred with a notable decrease in pentose pathway flux and the operation of the TCA cycle in a reductive mode. Simultaneous consumption of residual sugar and organic acids took place when oxygen sufficient conditions were restored though amino acids yields were significantly perturbed.