Transgenic poplars: expression of chimeric genes using four different constructs

• DOI: 10.1007/bf00232166
• Publication Date: 2004-09-07T07:27:36Z
• Authors: JeanCharles Leple; AnaCristinaMirand...; MarieFrance Michel; Francis Delmotte; Lise Jouanin
• Keywords: Callus; Octopine; Kanamycin

Leaf or stem explants of a hybrid poplar clone (Populus tremula X Populus alba), sensitive to Agrobacterium tumefaciens, were co-cultivated either by an octopine or a nopaline disarmed A. tumefaciens modified strain. Transformed poplar shoots were readily regenerated from explants. The protocol was improved using the nopaline disarmed strain C58/pMP90 with the binary vector pBI121. This protocol was then used to test three other vectors. The first one, possessing a nptII gene fused to the CaMV 19S promoter, permitted regeneration of transformed shoots in presence of 50 to 100 mg/l kanamycin. The two other vectors carried an additional nptII gene under the control of the CaMV 35S or CaMV 35S promoter with a double enhancer sequence (CaMV 70). CaMV 70 promoter provided consistently higher level of gene expression than the other promoters in both callus and leaf tissues.