Nitrogen fixation (acetylene reduction) associated with the living coral Acropora variabilis

• DOI: 10.1007/bf00431399
• Publication Date: 2004-11-16T05:44:31Z
• Authors: W. M. Williams; A. B. Viner; W. J. Broughton
• Keywords: Acropora; Acetylene

To estimate N2-fixation, acetylene reduction assays were carried out on portions of the branches of the coral Acropora variabilis from the west coast of Malaysia. In some experiments, a sub-surface incubation apparatus was employed that was designed to keep the coral fragments near to their natural depth of occurrence. Other shipboard experiments used metabolic inhibitors to investigate the class of organism reducing acetylene. Stumps of coral gave the highest rates of activity, probably attributable to loosely associated cyanophytes. Coral tips also reduced acetylene at relatively high rates; reduction was enhanced in light by increased CO2 concentration and decreased O2 tensions indicative of photosynthetic bacteria. Algal material was not obvious on the tip surfaces and so the active organism was probably more integral to the coral structure than it was in the stumps. Maximum rates of acetylene reduction measured translated to 2.5 mg N2 fixed per outcrop per day.