(Na+ −K+) dependent ATPase in mouse submaxillary gland

• DOI: 10.1007/bf00585848
• Publication Date: 2004-11-23T02:35:36Z
• Authors: Nebojša Avdalović; Zvonimir Kaić; Ivan Damjanov; Davor Solter
• Keywords: Submaxillary gland

The influences of castration and of testosterone administration on (NaK)-ATPase in mouse submaxillary gland has been studied. Electron microscopical and histochemical data showing a profound change in the structure of the granular tubules after castration are also presented. Whereas testosterone administration is followed by a proliferation of the rough and smooth endoplasmic reticulum in the cells of the granular tubules, castration results in an opposite change. After castration, alkaline phosphatase, which is primarily localized in the basal membranes of the granular tubules, is drastically reduced. The tissue was fractionated, by the procedure of Katz and Epstein [15], and microsomal membranes were isolated by a modification of the procedure described by Schwartz et al.[29]. Plasma membranes were isolated by the method of Henning et al.[9].