Determination of gastrodin,p-hydroxybenzyl alcohol, vanillyl alcohol,p-hydroxylbenzaldehyde and vanillin in tall gastrodia tuber by high-performance liquid chromatography

• DOI: 10.1007/bf02491665
• Publication Date: 2006-09-19T05:53:08Z
• Authors: C. L. Liu; M. C. Liu; P. L. Zhu
• Keywords: Gastrodin; Gastrodia elata

A high-performance liquid chromatographic method for the separation and determination of gastrodin,p-hydroxybenzyl alcohol, vanillyl alcohol,p-hydroxylbenzaldehyde and vanillin in extract of Chinese herbal medicine tall gastrodia tuber (Chinese name: Tianma) was established. The chromatographic conditions were optimized by means of computer-assisted method development technique. Dry-Lab software was used to model the retention behavior of the compounds as a function of gradient conditions, based on the data from two scouting gradient runs. Under the optimized conditions: column, Kromasil-C18, 5 μm, 15×0.46-cm; solvent A, water; solvent B, methanol; gradient, 5/44/65/65% B at 0/9/12/15 min; flow rate, 1.00 mL min−1; temperature, ambient, the quality of tall gastrodia tubers from different sources and tianma injection were examined.