Identification of CD36 as a new interaction partner of membrane NEU1: potential implication in the pro-atherogenic effects of the elastin receptor complex

• DOI: 10.1007/s00018-018-2978-6
• Publication Date: 2018-11-29T05:48:26Z
• Authors: Charlotte Kawecki; Olivier Bocquet; Christian E. H. S...; Andrea Heinz; Christian Ihling; Amandine Wahart; Béatrice Romier; Amar Bennasroune; Sébastien Blaise; Christine Terryn; Kenneth J. Linton; Laurent Martiny; Laurent Duca; Pascal Maurice
• Keywords: CD36; Sialidase; Scavenger Receptor; Catabolism

In addition to its critical role in lysosomes for catabolism of sialoglycoconjugates, NEU1 is expressed at the plasma membrane and regulates a myriad receptors by desialylation, playing key many pathophysiological processes. Here, we developed proteomic approach dedicated purification identification LC–MS/MS interaction partners human macrophages. Already known were identified as well several new candidates such class B scavenger receptor CD36. Interaction between CD36 was confirmed complementary approaches. We showed that elastin-derived peptides (EDP) desialylate this effect blocked V14 peptide, which blocks bioactive EDP elastin complex (ERC). Importantly, also increased uptake oxidized LDL macrophages both peptide sialidase inhibitor 2-deoxy-2,3-didehydro-N-acetylneuraminic acid (DANA). These results demonstrate, first time, binding ERC indirectly modulates sialylation level through sialidase. effects could contribute previously reported proatherogenic add dimension regulation biological processes NEU1.