Characterisation and hierarchy of the spermatogonial stem cell compartment in human spermatogenesis by spectral cytometry using a 16-colors panel

• DOI: 10.1007/s00018-024-05496-6
• Publication Date: 2024-12-26T18:16:01Z
• Authors: C. Lapoujade; M. Blanco; M. Givelet; A. S. Gille; I. Allemand; L. Lenez; N. Thiounn; S. Roux; J. P. Wolf; C. Patrat; L. Riou; V. Barraud-Lange; P. Fouchet
• Keywords: Compartment (ship); Cytometry

About one in six couples experience fertility problems, and male infertility accounts for about half of these cases. Spermatogenesis originates from a small pool spermatogonial stem cells (SSCs), which are interest the treatment but remain poorly characterised humans. Using multiparametric spectral flow cytometric analysis with 16-colours (16-C) panel cell markers, we identify novel markers SSCs provide insights into unravelling resolving heterogeneity human cells. This 16-C allowed identification primitive state β2M−CD51/61−ITGA6+SSEA4+TSPAN33+THY1+CD9medEPCAMmedCD155+CD148+CD47highCD7high phenotype, profile close to most states 0 SSC1-B previously defined by sc-RNAseq approach. The hierarchy events progenitor compartment spermatogenesis can be delineated. highlights importance multi-parametric cytometry in-depth characterisation testicular should help overcome lack knowledge, that hinders understanding regenerative potential SSCs, is critical parameter successful development new SSCs-based therapies.