Design, synthesis and biological evaluation of novel indolinedione–coumarin hybrids as xanthine oxidase inhibitors

• DOI: 10.1007/s00044-020-02589-2
• Publication Date: 2020-06-16T05:02:16Z
• Authors: Harmandeep Kaur G...; Kavita Bhagat; Atamjit Singh; Nitish Kumar; Arshmeet Kaur; Akriti Sharma; Shilpa Heer; Harbinder Singh; Jatinder Vir Singh; Preet Mohinder S....
• Keywords: IC50; Xanthine; Xanthine oxidase inhibitor

A library of indolinedione–coumarin hybrid molecules was rationally designed and synthesized against hyperuricemia. All of the synthesized hybrid molecules were tested to check their inhibitory activity against xanthine oxidase enzyme by using a spectrophotometric assay. The results revealed that the compound showed IC50 values within the range of 6.5–24.5 µM amongst which compound K-7 was found to be endowed with the most potent IC50 value against xanthine oxidase enzyme. Kinetic studies were also performed to check the mode of inhibition of most potent compound K-7, which revealed its mixed-type inhibition behavior. Structure-activity relationships revealed that electron-donating groups and small alkyl chains between the two active scaffolds might be beneficial in inhibiting xanthine oxidase enzyme. It was also shown that various electrostatic interactions stabilized the compound K-7 within the active site of xanthine oxidase enzyme, which confirmed that it can completely block its catalytic active site. Thus, K-7 is regarded as a potent xanthine oxidase inhibitor and can be served as a promising molecular architectural unit for anti-hyperuricemic drug design.