Modulation of radiation-induced oral mucositis (mouse) by dermatan sulfate: effects on differentiation processes

• DOI: 10.1007/s00066-019-01532-8
• Publication Date: 2019-11-08T16:18:39Z
• Authors: Nilsu Cini; Sylvia Gruber; Zumre Arican Alic...; Wolfgang Dörr
• Keywords: Mucositis; Dermatan sulfate; Amifostine

During head and neck cancer radiotherapy, oral mucositis is the most frequent early side effect. Systemic dermatan sulfate (DS) administration has been shown to significantly decrease mucosal radiation reactions during daily fractionated irradiation (IR) in an established mouse model. The aim of this study was investigate mechanism epithelial differentiation process, IR alone combination with DS treatment same model.Fractionated 5 × 3 Gy/week given snouts mice over two weeks, either or 4 mg/kg (IR + DS). Groups (n = 3) were sacrificed every second day course 14 days both experimental arms. Their tongue excised subjected immunohistochemical processing.In p16 analysis as a proliferation marker, difference between germinal (proliferation) layer not significant, stimulating process. For p21 marker on functional (differentiation) layer, arms indicating that inhibited In cytokeratin (CK) indicator cellular skeletal integrity, percentage antibody-positive cells above normal level superior arm.The protective activity DS, instead proliferation, based prevention cell loss by effects leading inhibition increase expression mechanical strength intercellular junctions.